DNA Separations by Slab Gel, and Capillary Electrophoresis: Theory and Practice
- 1 January 1995
- journal article
- research article
- Published by Taylor & Francis in Separation and Purification Methods
- Vol. 24 (1) , 1-118
- https://doi.org/10.1080/03602549508014343
Abstract
In this review we evaluate existing experimental methods for the electrophoretic size separation of nucleic acids, including both slab gel electrophoresis and capillary electrophoresis (CE). First, we briefly discuss some important applications of DNA electrophoresis in molecular biology. We then focus on the theory and the practice of DNA separations by slab gel electrophoresis and CE, emphasizing the different practical limitations of the two techniques. In particular, we review new developments in CE, a technique which is evolving rapidly.Keywords
This publication has 320 references indexed in Scilit:
- The use of coated and uncoated capillaries for the electrophoretic separation of DNA in dilute polymer solutionsElectrophoresis, 1995
- Electroosmosis of polymer solutions in fused silica capillariesElectrophoresis, 1994
- Ultrasensitive plasmid mapping by high performance capillary electrophoresisElectrophoresis, 1993
- A model for the mobility of single‐stranded DNA in capillary gel electrophoresisElectrophoresis, 1993
- Why can we not sequence thousands of DNA bases on a polyacrylamide gel?Electrophoresis, 1992
- Electrophoretic separation of S. Pombe chromosomes in polyacrylamide solutions using a constant fieldBiochemical and Biophysical Research Communications, 1991
- Zone broadening in electrophoresis with special reference to high‐performance electrophoresis in capillaries: An interplay between theory and practiceElectrophoresis, 1990
- On-column radiometric detector for capillary isotachophoresisJournal of Radioanalytical and Nuclear Chemistry, 1989
- Free zone electrophoresisChromatographic Reviews, 1967
- Agarose as an anticonvection agent in zone electrophoresisBiochimica et Biophysica Acta, 1961