Metabolism of cyclooxygenase and lipoxygenase products by 15-prostaglandin dehydrogenase from human HL-60 leukemia cells
- 1 August 1987
- journal article
- Published by Springer Nature in Inflammation Research
- Vol. 21 (3-4) , 397-399
- https://doi.org/10.1007/bf01966527
Abstract
A number of prostaglandins and mono-hyroxylated fatty acids were investigated as substrates for NAD-dependent 15-prostaglandin dehydrogenase (15-PGDH) obtained from human HL-60 cells. Lipoxygenase and cyclooxygenase-derived substrates possessing an w-6 hydroxyl moiety (15-HETE, 13-HODD and HHT) were metabolized to corresponding keto derivatives at a rate comparable to that for prostaglandins E2 and F2α. 12-HETE was a poor substrate and 5-HETE was not metabolized. The w-6 hydroxy fatty acids inhibited the metabolism of PGE2 and therefore they may play a role in modulating bioactive prostaglandin levels.Keywords
This publication has 7 references indexed in Scilit:
- Identification of 12-keto-5,8,10-heptadecatrienoic acid as an arachidonic acid metabolite produced by human HL-60 leukemia cellsBiochemical Pharmacology, 1987
- 12-L-hydroxy-5,8,10-heptadecatrienoic acid (HHT) is an excellent substrate for NAD+-dependent 15-hydroxyprostaglandin dehydrogenaseBiochemical and Biophysical Research Communications, 1985
- Metabolism of prostaglandin E2 in guinea pig liver. II. Pathways in the formation of the major metabolites.1971
- Metabolism of Prostaglandin E2 in Guinea Pig LiverPublished by Elsevier ,1971
- The Biological Activities of Three Metabolites of Prostaglandin E1Acta Physiologica Scandinavica, 1966
- Prostaglandins and Related FactorsPublished by Elsevier ,1964
- PROSTAGLANDINS + RELATED FACTORS .28. METABOLISM OF PROSTAGLANDIN E1 IN GUINEA PIG LUNG - STRUCTURES OF 2 METABOLITES1964