Preferential loss or inactivation of chromosome Z4APRT allele in CHO cells
- 1 July 1989
- journal article
- research article
- Published by Springer Nature in Somatic Cell and Molecular Genetics
- Vol. 15 (4) , 271-277
- https://doi.org/10.1007/bf01534966
Abstract
In CHO cells, heterozygotes for the adenine phosphoribosyltransferase (APRT)locus arise spontaneously at high frequencies. However, such heterozygotes always yield APRT − mutants at low spontaneous rates. In an attempt to determine whether differences in the genomic environments of the two CHO APRTalleles might render one gene more susceptible to high-frequency spontaneous inactivation or deletion, we have mapped the functional APRTallele in four different spontaneous APRTheterozygotes. In each case, the functional APRTgene was found to reside on the Z7 chromosome; it was always the Z4 APRTallele that had been lost or inactivated. Two of these heterozygotes were shown to be physically hemizygous while the other two retained two copies of the APRT gene, indicating that the high-frequency event can involve either spontaneous deletion or inactivation.This publication has 21 references indexed in Scilit:
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