PRESENCE OF STEROID-RECEPTORS IN HUMAN SOFT-TISSUE SARCOMAS OF DIVERSE HISTOLOGICAL ORIGIN

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  • 1 January 1980
    • journal article
    • research article
    • Vol. 40  (3) , 861-865
Abstract
The incidence of cytosol receptors for androgen, estrogen, glucocorticoid and progesterone was determined in a series of histologically diverse soft tissue sarcomas. Receptor binding was characterized in tumor specimens from 29 adult patients (14 female, 15 male) obtained during surgery. Nineteen (66%) tumor cytosols bound at least 1 steroid. Seven (24%) had more than 1 receptor. Ten of 23 (43%) cytosols assayed for androgen binding were positive (Kd 0.97 .+-. 0.23 .times. 10-9 M; 60.6 .+-. 25.9 f[femto]mol/mg protein), 7 of 29 (24%) for estrogen (Kd 1.00 .+-. 0.19 .times. 10-9 M; 33.2 .+-. 10.9 fmol/mg protein), 9 of 26 (35%) bound glucocorticoid (Kd 4.22 .+-. 1.77 .times. 10-9 M; 231.0 .+-. 155.0 fmol/mg protein), and 1 of 28 (4%) bound progesterone (Kd 0.41 .times. 10-9 M; 18.7 fmol/mg protein). Density gradient analysis suggested that androgen and estrogen binding was located predominantly in the 6S and 7-8S regions, respectively, whereas receptor for glucocorticoid sedimented at 8S. Steroid binding was not related to patient age. Estrogen-positive (71%) and glucocorticoid-positive (78%) cytosols appeared predominantly in tumors from female patients. Receptor distribution also appeared to be correlated to tumor histogenetic origin.