A reaction associated with nonenzymatic binding in the reticulocyte transfer system.
- 1 June 1967
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 57 (6) , 1817-1824
- https://doi.org/10.1073/pnas.57.6.1817
Abstract
A reaction of the reticulocyte transfer system leading to resistance to inhibition by NaF and associated with chain initiation is described. Rabbit liver S-RNA was used. The reaction was dependent upon 1 or more soluble factors in added enzyme fractions and upon sulfhydryl compounds. The reaction was dependent upon but distinguishable from nonenzymatic binding. It was not dependent upon added GTP. It is suggested that the formation of an active complex involving ribosomes, messenger RNA, amino-acyl-sRNA, and 1 or more soluble transfer enzymes is involved.This publication has 11 references indexed in Scilit:
- TRANSLATION OF THE GENETIC MESSAGE, II. EFFECT OF INITIATION FACTORS ON THE BINDING OF FORMYL-METHIONYL-TRNA TO RIBOSOMESProceedings of the National Academy of Sciences, 1967
- A factor from E. coli required for the translation of natural messenger RNABiochemical and Biophysical Research Communications, 1966
- The mechanism of sodium fluoride and cycloheximide inhibition of hemoglobin biosynthesis in the cell-free reticulocyte systemJournal of Molecular Biology, 1966
- Differential inactivation of soluble reticulocyte transfer factors with N-ethylmaleimideBiochemical and Biophysical Research Communications, 1966
- A Factor from Escherichia coli Concerned with the Stimulation of Cell-Free Polypeptide Synthesis by Exogenous Ribonucleic Acid. II. Characteristics of the Reaction Promoted by the Stimulation Factor*Biochemistry, 1966
- A Factor from Escherichia coli Concerned with the Stimulation of Cell-Free Polypeptide Synthesis by Exogenous Ribonucleic Acid. I. Evidence for the Occurrence of a Stimulation Factor*Biochemistry, 1966
- NaF inhibition of the initial binding of aminoacyl-sRNA to reticulocyte ribosomes.Proceedings of the National Academy of Sciences, 1966
- Comparison of guanosine triphosphate split and polypeptide synthesis with a purified E. coli system.Proceedings of the National Academy of Sciences, 1966
- MECHANISM OF PEPTIDE BOND FORMATION IN POLYPEPTIDE SYNTHESISProceedings of the National Academy of Sciences, 1964
- Participation of two enzyme fractions in the transfer of amino acids from amino acyl-RNA to reticulocyte ribosomesBiochimica et Biophysica Acta, 1961