Expression of human thromboxane synthase using a baculovirus system
- 22 February 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 318 (1) , 91-94
- https://doi.org/10.1016/0014-5793(93)81335-w
Abstract
Human thromboxane (TX) synthase (EC 5.3.99.5) was produced by the baculovirus expression system using cDNA encoding human TX synthase [(1991) Biochem. Biophys. Res. Commun. 78, 1479-1484]. A recombinant baculovirus TXS7 was expressed in Spodoptera frugiperda Sf9 insect cells. The expressed protein was recognized by monoclonal antibody, Kon 7 raised against human TX synthase [(1990) Blood 76, 80-85]. The recombinant TX synthase catalyzed the conversion of prostaglandin (PG) H2 to TXA2 and 12-hydroxy-heptadecatrienoic acid (HHT). Both conversions of PGH2 to TXA2 and HHT by the expressed TX synthase were completely inhibited by a specific TX synthase inhibitor, OKY-046 (5 μM)Keywords
This publication has 16 references indexed in Scilit:
- Molecular cloning of human platelet thromboxane a synthaseBiochemical and Biophysical Research Communications, 1991
- Expression and characterization of a rabbit liver cytochrome P450 belonging to P450IIB subfamily with the aid of the baculovirus expression vector systemBiochemical and Biophysical Research Communications, 1991
- Immunoaffinity purification of human thromboxane synthaseArchives of Biochemistry and Biophysics, 1990
- Cloning of human gene encoding prostaglandin endoperoxide synthase and primary structure of the enzymeBiochemical and Biophysical Research Communications, 1989
- Novel exogenous heme-dependent expression of mammalian cytochrome P450 using baculovirusArchives of Biochemistry and Biophysics, 1989
- ARACHIDONIC ACID METABOLISMAnnual Review of Biochemistry, 1986
- Highly selective inhibitors of thromboxane synthetase. 1. Imidazole derivativesJournal of Medicinal Chemistry, 1981
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Thromboxanes: a new group of biologically active compounds derived from prostaglandin endoperoxides.Proceedings of the National Academy of Sciences, 1975
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970