Nucleotide sequence of the 16S – 23S spacer region in an rRNA gene cluster from tobacco chloroplast DNA
Open Access
- 1 January 1982
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 10 (8) , 2665-2676
- https://doi.org/10.1093/nar/10.8.2665
Abstract
The nucleotide sequence of a spacer region between 16S and 23S rRNA genes from tobacco chloroplasts has been determined. The spacer region is 2080 bp long and encodes tRNAIle and tRNAAla genes which contain intervening sequences of 707 bp and 710 bp, respectively. Strong homology between the two intervening sequences is observed. These spacer tRNAs are synthesized as part of an 8.2 kb precursor molecule containing 16S and 23S rRNA sequences.Keywords
This publication has 24 references indexed in Scilit:
- Sequencing of the 16S–23S spacer in a ribosomal RNA operon of Zea mays chloroplast DNA reveals two split tRNA genesCell, 1981
- Overlapping divergent genes in the maize chloroplast chromosome and in vitro transcription of the gene for tRNA HisProceedings of the National Academy of Sciences, 1981
- Analysis of a Drosophila tRNA gene cluster: Two tRNALeu genes contain intervening sequencesCell, 1981
- The structure and evolution of the human β-globin gene familyCell, 1980
- Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.Proceedings of the National Academy of Sciences, 1980
- Sequencing of 16S–23S spacer in a ribosomal RNA operon of Euglena gracilis chloroplast DNA reveals two tRNA genesNature, 1980
- Cloning and characterization of 4.5S and 5S RNA genes in tobacco chloroplastsGene, 1980
- Splicing of yeast tRNA precursors: structure of the reaction intermediatesCell, 1979
- Genetic studies of the lac repressorJournal of Molecular Biology, 1978
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977