Resistotyping of campylobacters: fulfilling a need
- 1 April 1996
- journal article
- research article
- Published by Cambridge University Press (CUP) in Epidemiology and Infection
- Vol. 116 (2) , 169-175
- https://doi.org/10.1017/s0950268800052407
Abstract
SUMMARY A 9-month trial of a simple typing scheme for ‘thermophilic’ enteric campylobacter isolates at a large Public Health Laboratory is described. Resistotyping was performed with six agents in a method modified by Bolton and colleagues from an earlier scheme, and biotyping was performed by a modified Lior scheme involving three tests. Reproducibility was excellent in both schemes, with test variation < 2%. Five household clusters and one larger presumptive milk-borne outbreak were identified in this scheme, and confirmed in pyrolysis mass spectrometry. The 328 isolates from new patients, excluding duplication from these clusters, were divided into 35 resistotypes with the largest group comprising 22% of isolates. In combined bio- and resistotyping, 86 types were found, with the largest group comprising 9·5% of isolates. The results are contrasted with salmonella sero- and phage-typing, where, on the same basis, the 176 isolates in the same period were divided into 40 groups, with the largest comprising 45% of isolates. Resistotyping, with or without additional biotyping, proved to be a convenient, simple, rapid, highly discriminatory, reproducible and inexpensive method well suited to use in local laboratories. It is a strong candidate for first-line national and local surveillance of campylobacter infections, fulfilling a need for monitoring of this important cause of enteric disease.Keywords
This publication has 13 references indexed in Scilit:
- Pyrolysis typing of isolates from a recurrence of systemic cryptococcosisJournal of Medical Microbiology, 1994
- Ribosomal RNA gene restriction fragment diversity amongst Lior biotypes and Penner serotypes of Campylobacter jejuni and Campylobacter coliFEMS Microbiology Letters, 1992
- RAPD analysis ofCampylobacterisolates: DNA fingerprinting without the need to purify DNALetters in Applied Microbiology, 1992
- Jackdaws and magpies as vectors of milkborne human campylobacter infectionEpidemiology and Infection, 1991
- Bird attack on milk bottles: possible mode of transmission of Campylobacter jejuni to manThe Lancet, 1990
- DNA restriction digest and ribosomal RNA gene patterns ofCampylobacter jejuni: a comparison with bio-, sero-, and bacteriophage-types of United Kingdom outbreak strainsEpidemiology and Infection, 1990
- Pyrolysis-mass spectrometry (Py-MS) for the rapid epidemiological typing of clinically significant bacterial pathogensJournal of Medical Microbiology, 1990
- Campylobacter biotyping scheme of epidemiological value.Journal of Clinical Pathology, 1984
- Differentiation of enteropathogenic Campylobacter.Journal of Clinical Pathology, 1980
- Serotyping of Campylobacter jejuni/coli.Journal of Clinical Pathology, 1980