Neuronal Protection in Stroke by an sLe x -Glycosylated Complement Inhibitory Protein
- 23 July 1999
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 285 (5427) , 595-599
- https://doi.org/10.1126/science.285.5427.595
Abstract
Glycoprotein adhesion receptors such as selectins contribute to tissue injury in stroke. Ischemic neurons strongly expressed C1q, which may target them for complement-mediated attack or C1qRp-mediated clearance. A hybrid molecule was used to simultaneously inhibit both complement activation and selectin-mediated adhesion. The extracellular domain of soluble complement receptor–1 (sCR1) was sialyl Lewis x glycosylated (sCR1sLex) to inhibit complement activation and endothelial-platelet-leukocyte interactions. sCR1 and sCR1sLexcolocalized to ischemic cerebral microvessels and C1q-expressing neurons, inhibited neutrophil and platelet accumulation, and reduced cerebral infarct volumes. Additional benefit was conferred by sialyl Lewis x glycosylation of the unmodified parent sCR1 molecule.Keywords
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