Messenger RNA for Globin in the Postribosomal Supernatant of Rabbit Reticulocytes
- 1 June 1972
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 69 (6) , 1425-1428
- https://doi.org/10.1073/pnas.69.6.1425
Abstract
10S RNA has been isolated from the postribosomal supernatant of rabbit reticulocyte lysate. This RNA is associated with protein in a ribonucleoprotein particle that sediments at approximately 20S. The 10S RNA extracted from this particle codes predominantly for alpha globin chains when it is translated by a mouse ascites cell-free extract. The product of the cell-free synthesis has been characterized by column chromatography and electrophoresis of the tryptic peptides. About 20% of the total alpha chain mRNA is present in the postribosomal supernatant, whereas beta chain mRNA is almost exclusively associated with polyribosomes.Keywords
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