Regulation of metabolic transcriptional co‐activators and transcription factors with acute exercise

Abstract
SPECIFIC AIMSTranscription factors and their target genes, especially those involved with PPAR families, can be regulated directly via increases in free fatty acids (FA). As physical exercise stimulates lipolysis and increases circulatory FFA levels, the effect of exercise per se on the induction of metabolic transcription factors can be biased by elevated FFA levels. To better understand the pathways involved in eliciting the adaptive response to exercise, the aim of the present study was to investigate the effect of acute endurance exercise on the regulation of the mRNA of several key metabolic transcriptional coactivators, including PGC-1α and PRC, transcription factors PPARα, β/δ, and γ, RXR, SREBP-1c and FKHR, and to delineate the effect of exercise from the effect of elevated levels of circulating FFA. To this end, exercise was performed once in the fasted state and once in the glucose fed state.PRINCIPAL FINDINGS1. The effect of FFA levels on the regulation of transcription factorsUnder resting pre...