Combination of Size Exclusion and Adsorption Phenomena on a Hydrophilic Polymer Gel Column Using Organic Solvents

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Abstract
Elution behavior of alkylbenzenes, phthalate esters, oligostyrenes, and prepolymers of epoxy resins and methylated melamine-formaldehyde resins has been investigated on a column packed with hydrophilic polyhydroxyethyl methacrylate gels using tetrahydrofuran (THF), methanol, and mixtures of THF and methanol or n-hexane as the mobile phases. Size-exclusion effect was predominant when THF was used as the mobile phase and adsorption interactions between solutes and the gels superimposed the size-exclusion effect when methanol or mixtures of THF and methanol or n-hexane was the mobile phase. The gel-THF system was assumed to be SEC. The addition of methanol or n-hexane to THF in the mobile phase retarded the elution of solutes. The combination of size exclusion and adsorption modes in the same column was possible by changing composition of the mobile phase and resolution of some peaks was improved than by SEC alone. The peak identification by the present system is also improved than by SEC.

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