The cytochromes of isolated chloroplasts

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Abstract
Suspensions of chloroplasts extracted from leaves of Vicia faba L. were purified to the extent that no cytoplasmic impurities were detectable in electron micrographs. These suspensions contained cytochrome bg and cyto-chrome f and none of the cytochromes found in mitochondria. Both cytochromes present were limited to the lamellae of the grana, i.e. to the same site as the chlorophylls. The molar ratios chlorophyll achlorophyll b:cytochrome b6:cytochrome f were estimated at about 460:260:2.5:1. It is suggested that the cytochromes, possibly a single molecule each of cytochrome b6 and cytochrome f, may form the node around which the "photosynthetic unit" is organized.