HUMAN URINARY-BLADDER CARCINOMA CELL LINE (T24) IN LONG-TERM CULTURE - CHROMOSOMAL STUDIES ON A WILD POPULATION AND DERIVED SUBLINES

  • 1 January 1977
    • journal article
    • research article
    • Vol. 24  (3) , 319-326
Abstract
Karyotype changes of the human urinary bladder carcinoma T24 cell line were studied in the course of 5 yr in vitro cultivation. The modal number of chromosomes gradually shifted from hypotetraploidy to triploidy during long-term cultivation. The number of endoreduplications decreased simultaneously and minute chromosomes occurring in a number of 4-5 in early passages appeared only rarely in later passages. Using conventional Giemsa staining, persistent marker chromosomes were detected in the wild population as well as in the 8 sublines derived from T24 cells by cloning. The marker chromosomes were metacentrics, subtelocentrics, telocentrics and long acrocentrics. Occasionally, dicentrics, double minutes, breaks and pulverization were found. The most characteristic marker was the metacentric chromosome the length of which corresponded approximately to the arm length of chromosome no. 1. Metacentric chromosomes in a number of 1-3 were present in 100% of T24 cells of the wild population and derived sublines at all passage levels examined.

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