Capillary gas chromatographic/mass spectrometric analysis of abnormal metabolites in hypoglycin-treated rat urine

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Abstract
Numerous abnormal metabolites were identified in large amounts in the urine of hypoglycin‐treated rats using capillary gas chromatography/mass spectrometry‐computer analysis. These metabolites are not detectable in significant amounts in normal rats' urine. Ten of them have not been previously associated with hypoglycin administration: these are several hydroxy compounds, including those from the valine and isoleucine pathways, 2‐oxo‐adipic acid, n‐butyrylglycine and isovaleryl glucuronide. These results indicate that the pathways of isoleucine and valine netabolism are inhibited at their respective acyl‐CoA dehydrogenation steps, as is the case for fatty acid, leucine and lysine metabolism, as previously shown. The mass spectra of the trimethylsilyl derivatives of cis, cis‐4,7‐decadiene‐1,10‐dioic, cis‐4‐decene‐1,10‐dioic, cis‐4‐octene‐1,8‐dioic acids, and (methylenecyclopropyl)acetylglycine, which were previously identified using nuclear magnetic resonance and oxidative cleavage or acid hydrolysis, are presented for the first time.

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