CELL LOSS FROM THREE ESTABLISHED LINES OF THE C3H MOUSE MAMMARY TUMOR: A COMPARISON OF THE125I-UdR AND THE3H-TdR-AUTORADIOGRAPHIC METHODS

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Abstract
The 125I-UdR [125I-iododeoxyuridine] method for measuring cell loss from solid tumors was evaluated. The rate of tumor cell loss from 3 established lines (S102F, S102S and Slow) of the C3H mouse mammary tumor was determined by the 125I-UdR method and results were compared to estimates for cell loss as determined by the combined approach of cellular 3H-TdR [3H-thymidine] autoradiography and volumetric growth-rate determinations. This detailed comparison shows that the 2 methods complement each other but cannot substitute for one another because they give different quantitative information. The combined approach measures the flow of viable cells, as determined morphologically, from the proliferating compartment to the quiescent compartment, the quiescent compartment out of the tumor, etc., but does not evaluate the flow of degenerate cells or acellular (necrotic) debris. The 125I-UdR method indicates the net flow of intact cells and/or dead cells as well as debris from the tumor as the 125I-labeled material is lost from the tumor, but gives limited internal information. Depending on the specific experiment, one or the other of the methods can be chosen to answer the question. Perhaps both would be desirable at times; in most cases, one could not be substituted for the other. The data from the Slow tumors also indicate that in certain tumors, the quantitative information from the 125I-UdR method may be quite limited, i.e., the confidence limits within an experiment as well as the replication error between experiments may be high.

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