Determining the location of hydrophobic spin traps within liposomes
- 1 January 1994
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Journal of the Chemical Society, Perkin Transactions 2
- No. 6,p. 1229-1232
- https://doi.org/10.1039/p29940001229
Abstract
We have recently reported on an initial study of free radical penetration into liposomal membrane using two new hydrophobic spin traps, namely 2,2-dimethyl-4-phenyl-2H-imidazole 1-oxide (DMPIO) and its 2-methyl-2-nonyl analogue (MNPIO). The radical trapping data was most readily resolved by positing that only MNPIO is lodged completely within the lipid bilayer; the N-oxide group of DMPIO, on the other hand, is actually located at or near the lipid/water interface, available for interaction with aqueous phase radicals. We have been able to confirm these results by using the 13C chemical shifts of these spin traps as a gauge with which to measure the polarity of the microenvironment experienced by the carbons. As expected, the spin traps experience a gradient of solvent polarities, increasing as one goes from deep within the lipid bilayer out toward the aqueous phase.Keywords
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