Proteolytic Enzyme System of Skin. VI. Enzyme Patterns in Various Animal Species.

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Abstract
Fresh skin extracts of various animals have been surveyed for their ability to hydrolyze N-acetyl-L-tyrosine ethyl ester (ATEE), L-tyrosine ethyl ester (TEE), L-phenylalanine ethyl ester (PEE), and casein. ATEEase activity was found in extracts of rat, guinea pig, sheep, and monkey skin but was absent in rabbit, hog, cow, and chicken skin extracts. Only rat skin ATEEase activity was depressed in the presence of a blood inhibitor. Activity toward TEE and PEE was somewhat more prevalent with rat, guinea pig, rabbit, hog, sheep, and monkey skin extracts exhibiting this activity. Comparable activity was absent in cow and chicken skin extracts. Proteinase activity, using casein as the substrate, was found in rat, guinea pig, and monkey skin extracts, and absent in the other species examined. The proteinase activity was found to be capable of ionic strength-dependent reversible inactivation. The data obtained have been interpreted in terms of conformity to results previously obtained with extracts of rat skin acetone powder.