The Effects of Radioiodination and Fluorescent Labelling on Albumin
- 1 January 1981
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 11 (2) , 111-138
- https://doi.org/10.1080/00327488108064237
Abstract
The preparation and characterization of fluorescamine-, fluorescein isothiocyanate (FITC)- and radioiodine-labeled bovine serum albumin [BSA] is critically evaluated. Electrophoretic mobility and ion-exchange chromatography, together with measures of degree of conjugation and SH content, are used to assess the changes due to conjugation. Fluorescamine labeling results in drastic changes in chromatographic behavior and electrophoretic mobility. FITC labeling also results in significant changes in chromatographic and electrophoretic properties. Radioiodination leads to minor changes in chromatographic properties and oxidation of SH groups, with little or no change in electrophoretic properties. All 3 labels have some degree of lability and show increased levels of free label with time, even after extensive initial purification. The 2 fluorescent labels and possibly the radioiodine labeling method used here are unsuitable for certain studies of BSA, such as its adsorption at solid-liquid interfaces.This publication has 63 references indexed in Scilit:
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