Sodium‐dependent isoleucine transport in the methanogenic archaebacterium Methanococcus voltae
- 30 January 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 166 (2) , 357-361
- https://doi.org/10.1016/0014-5793(84)80112-x
Abstract
Methanococcus voltae possesses a Na+‐dependent transport system for isoleucine which requires for optimum rates a CO2/H2 atmosphere. The K m for the system is 4.5 μM with a V max of 1.5 nmol·min−1·mg dry wt−1. Approximately 75% of the label can be released from the cell pool following short‐term experiments with gradients of isoleucine reaching 100 (in/out). Transport is inhibited by ionophores and N‐ethyl maleimide. Only valine and leucine effectively compete with isoleucine for transport.Keywords
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