Radioimmuntests für die Bestimmung von Lysergsäure und einfachen Lysergsäurederivaten

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Abstract
Radioimmunoassays for the quantitative and separate determination of lysergic acid and simple lysergic acid derivatives were developed. Lysergic acid was coupled to bovine serum albumin both by the Mannich and the mixed anhydrid reaction and antibodies against these 2 different conjugates were raised in rabbits. 3H-lysergic acid was synthesized by basic hydrolysis of 3H-ergotamine. The antibodies produced against the conjugate, prepared by the Mannich reaction, were very specific for lysergic acid and did not cross react with any of the other ergot alkaloids tested. The antibodies raised against the conjugate, prepared by the mixed anhydrid reaction, cross reacted with all simple lysergic acid derivatives and some clavines. The antibodies exhibited high affinity towards lysergic acid and its derivatives. K = 0.46 .times. 109 l/mol (specific antiserum), K = 3.76 .times. 109 l/mol (polyspecific antiserum). The measuring range was between 1-112 pmol lysergic acid (use of specific antiserum) and between 0.6-31 pmol ergometrine (use of polyspecific antiserum). With the specific lysergic acid radioimmunoassay, Caviceps purpurea sclerotia and sapophytic C. purpurea cultures were screened for free lysergic acid, while the ergometrine content of a saprophytic C. purpurea culture was optimized with the polyspecific lysergic acid radioimmunoassay.

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