Analysis of K99 plasmids from enterotoxigenic Escherichia coli
Open Access
- 1 January 1992
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 90 (2) , 141-146
- https://doi.org/10.1016/0378-1097(92)90618-x
Abstract
Evaluation of 9 wild-type K99 positive strains of Escherichia coli showed that each had a plasmid of approximately 87.8 kb that hybridized with two DNA probes specific for K99 genes. The K99 reference plasmid from E. coli also is 87.8 kb. Each of these strains had a conserved 7.15-kb Bam HI fragment that also hybridized to these probes. Several K99 negative mutants and three 3P− strains also contained K99 plasmids as well as the 7.15-kb Bam HI fragment. These results suggest that there is a conservation in size of the K99 plasmids of diverse strains.Keywords
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