Acid and alkaline phosphatases of Capnocytophaga species. I. Production and cytological localization of the enzymes

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Abstract
The 2 hydrolytic enzymes, acid (AcP; EC 3.1.3.2) and alkaline (AlP: EC 3.1.3.1) phosphatase, of the 3 type species of Capnocytophaga were examined. Both enzymes were produced constitutively, with their activity highest in C. ochracea strain 25. These 2 degradative enzymes (.apprx. 10% of the total activity) were released into the growth medium during the latter stages of growth, as soluble and membrane-bound enzymes. When grown in the presence of high concentrations of organic phosphates, the synthesis and expression of AcP and AlP was unaltered. Cyto- and immuno-chemical localization situated the phosphatases in the periplasmic space, at the cell surface and in membranous vesicles.