The IFNAR1 subunit of the type I IFN receptor complex contains a functional nuclear localization sequence
- 13 November 2004
- journal article
- Published by Wiley in FEBS Letters
- Vol. 578 (3) , 207-210
- https://doi.org/10.1016/j.febslet.2004.10.085
Abstract
A nuclear localization sequence (NLS) in the type II interferon (IFN) IFNγ, which is responsible for the nuclear translocation of both the ligand and the α-subunit (IFNGR1) of the receptor complex, has previously been characterized and its role in signaling examined in detail. We have now identified an NLS in the type I IFN receptor (IFNAR) common subunit IFNAR1 from humans and show that the human IFNAR1 subunit can translocate to the nucleus following human IFNβ stimulation. An NLS in human IFNAR1 is located in the extracellular domain of IFNAR1 within the sequence 382RKIIEKKT (numbered for the precursor form). Nuclear import by the NLS functions in a conventional fashion requiring cytosolic import factors, is energy-dependent and inhibited by the prototypical NLS of the SV40 large T-antigen. These studies provide a mechanism for nuclear import of IFNAR1, as well as for type I IFN ligands, and a starting point for studying an alternate role for IFNAR1 in nuclear signaling within the type I IFN systemKeywords
This publication has 33 references indexed in Scilit:
- Trafficking and signaling pathways of nuclear localizing protein ligands and their receptorsBioEssays, 2004
- Signal Transduction Mechanism of A Peptide Mimetic of Interferon-γBiochemistry, 2004
- Nuclear localization and function of polypeptide ligands and their receptors: a new paradigm for hormone specificity within the mammary gland?Breast Cancer Research, 2003
- Nuclear Translocation and Retention of Growth HormoneEndocrinology, 2003
- Nuclear localization of EGF receptor and its potential new role as a transcription factorNature Cell Biology, 2001
- SO MANY LIGANDS, SO FEW TRANSCRIPTION FACTORS: A NEW PARADIGM FOR SIGNALING THROUGH THE STAT TRANSCRIPTION FACTORSCytokine, 2001
- Angiotensin II-Induced Nuclear Targeting of the Angiotensin Type 1 (AT1) Receptor in Brain Neurons*Endocrinology, 1998
- The type-I interferon receptor. The long and short of itCytokine & Growth Factor Reviews, 1996
- Nuclear accumulation of interferon gamma.Proceedings of the National Academy of Sciences, 1994
- Transport of γ-interferon into the cell nucleus may be mediated by nuclear membrane receptorsBiochemical and Biophysical Research Communications, 1986