Estimation of Michaelis-Menten Constants in Microsomal Enzyme Systems by the Direct Linear Plot and Least Squares Regression

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Abstract
1. Data from in vitro liver microsomal metabolism of ethylmorphine, aniline and benzpyrene in male rats and male and female quokkas (Setonix Brachyurus) were fitted to the Michaelis-Menten equation by Wilkinson's non-linear regression procedure and Eisenthal and Cornish-Bowden's direct linear plot. 2. Ethylmorphine and aniline metabolism in the male quokka were non-ideal, but in all other instances there were no differences in the performance of the two methods.