A B72.3 Second-Generation-Monoclonal Antibody (CC49) Defines the Mucin-Carried Carbohydrate Epitope Galβ(1—3) [NeuAcα(2—6)]GalNAc

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Abstract
Mucus glycoproteins from bovine seminal plasma were demonstrated to express the MAbs CC49- and B72.3-defined epitopes on TAG 72 antigen. Inhibition studies with reductively cleaved mucin O-glycans from bovine seminal plasma and submaxillary glands revealed that CC49 binds specifically to a core-type sialyl-oligosaccharide alditol (fraction 2c), which could be defined with regard to its primary structure by FAB-and EI-mass spectrometry combined with methylation analysis. Structurally related alditols NeuAc.alpha.(2-6)-GalNAc-ol, Gal.beta.(1-3)GalNAc-ol, NeuAc.alpha.(2-3)-Gal.beta.(1-3) [NeuAc.alpha.(2-6)] GalNAc-ol, GlcNAc.beta.(1-3) [NeuAc.alpha.(2-6)] GalNAc-ol or NeuAc.alpha.(2-3) Gal.beta.(1-3)GalNAc-ol were not inhibitory.