Protein Nanoarrays Generated By Dip-Pen Nanolithography
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- 1 March 2002
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 295 (5560) , 1702-1705
- https://doi.org/10.1126/science.1067172
Abstract
Dip-pen nanolithography was used to construct arrays of proteins with 100- to 350-nanometer features. These nanoarrays exhibit almost no detectable nonspecific binding of proteins to their passivated portions even in complex mixtures of proteins, and therefore provide the opportunity to study a variety of surface-mediated biological recognition processes. For example, reactions involving the protein features and antigens in complex solutions can be screened easily by atomic force microscopy. As further proof-of-concept, these arrays were used to study cellular adhesion at the submicrometer scale.Keywords
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