Poly(ethylene glycol)-block-poly(l-lysine) Dendrimer: Novel Linear Polymer/Dendrimer Block Copolymer Forming a Spherical Water-Soluble Polyionic Complex with DNA
- 31 December 1998
- journal article
- research article
- Published by American Chemical Society (ACS) in Bioconjugate Chemistry
- Vol. 10 (1) , 62-65
- https://doi.org/10.1021/bc9800668
Abstract
Methoxypoly(ethylene glycol)-block-poly(l-lysine) dendrimer was designed to form a water-soluble complex with plasmid DNA. The copolymer was synthesized by the liquid-phase peptide synthesis method. It was characterized by 1H NMR and matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrum. Agarose gel electrophoresis and DNase I protection assay proved that this linear polymer/dendrimer block copolymer assembled spontaneously with plasmid DNA, forming a water-soluble complex which increased the stability of the complexed DNA. Atomic force microscopy of the complex was evaluated at various charge ratios showing that the copolymer/DNA complex was like a globular shape.Keywords
This publication has 2 references indexed in Scilit:
- Stability of Peptide-Condensed Plasmid DNA FormulationsJournal of Pharmaceutical Sciences, 1998
- Characterization of Vectors for Gene Therapy Formed by Self-Assembly of DNA with Synthetic Block Co-PolymersHuman Gene Therapy, 1996