MONOCLONAL-ANTIBODIES TO THE MURINE LY-2.1 CELL-SURFACE ANTIGEN

  • 1 January 1982
    • journal article
    • research article
    • Vol. 46  (1) , 135-144
Abstract
Six monoclonal anti-Ly-2.1 antibodies are described that arose from a single fusion using the spleen from a 129/ReJ mouse immunized with CBA/H thymus cells. The specificity was determined from the strain distribution and testing of congenic strains where all 6 monoclonal antibodies detected the Ly-2.1 alloantigen. The antibodies fall into 2 groups. Group I (4 antibodies) detected the expected number of Ly-2+ thymocytes and peripheral T cells; group II (2 antibodies) detected 10% fewer peripheral T cells. Functional studies demonstrated that the Ly-2.1 determinant detected by a group I antibody (49-31.1) was present on cytotoxic T cells (Ly-1+2+) on concanavalin A (Con-A)-induced suppressor T cells (Ly-1-2+), but absent from helper T cells (Ly-1+2-). One antibody (49-11.1) precipitated a 68,000-75,000 MW glycoprotein, which on reduction yielded 30,000 and 35,000 MW moieties. Thus, by functional, genetic and biochemical criteria these antibodies detected the Ly-2.1 specificity. In addition, a monoclonal, noncytotoxic, IgG1, anti-Thy-1.2 antibody is described.

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