Inhibition of human interferon-gamma expression by antisense oligodeoxynucleotides

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Abstract
We investigated 12 antisense oligodeoxynucleotides, complementary to different regions of the human interferon-γ (HuIFN-γ) gene, for their ability to inhibit HuIFN-γ production in cultures of single donor total leukocytes or lymphocytes (95% purity). Out of seven oligomers, specific for a sequence including the translation initiation codon, 15 to 21 nucleotides long, the one resulting in the greatest inhibition was the 16-mer. An inhibitory effect of 90% could be achieved when the oligomer was added to cultures of lymphocytes in separate doses. Three other 16-mers, complementary to a sequence in the 5′ noncoding region, in the coding region, or at the donor splice junction of the third intron respectively, were inhibitory to a lesser extent. Two 16-mers, one complementary to a sequence at the donor splice junction of the second intron and one specific for a sequence in the 3′ untranslated region, showed no effect. J. Leukoc. Biol. 55: 169–174; 1994.

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