Response of Isolated Leukocyte Lysosomes to Gamma Irradiation

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Abstract
Lysosomes isolated from rabbit poly-morphonuclear leukocytes were irradiated in vitro at 0[degree] to 4[degree]C with 1 to 50 krads of gamma-radiation and incubated for 1 hr at 0[degree] to 4[degree]C, 28[degree]C, or 37[degree]C. Irradiation of lysosome suspensions resulted in a rapid drop in their optical density (520 m[mu]). The rate at which thi fall occurred during the first 15 min. postirradiation was dose-dependent. The magnitude of the decrease in turbidity resulting from irradiation was less during incubation at 0[degree] to 4[degree]C than at higher temperatures; at all temperatures the effect showed a tendency to level off during the latter portion of the incubation period. Irradiated lysosome suspensions had increased amounts of protein in the granule-free suspending medium. At high doses this effect was greater, relative to controls, at 0[degree] to 4[degree]C than at higher incubation temperatures. Radiation may have produced some form of damage in or near the membrane of the isolated lysosome and subsequent stress (heating) resulted in an exaggerated response (fall in optical density). The leakage of protein, occurred in the absence of extreme stress and may represent a more sensitive indicator of lysosomal damage. The possible relationship of these data to the role of the lysosome in the initiation or exacerbation of cellular radiation damage was discussed.

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