Microcolumn Separations and the Analysis of Single Cells
- 6 October 1989
- journal article
- review article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 246 (4926) , 57-63
- https://doi.org/10.1126/science.2675314
Abstract
Capillary zone electrophoresis and open tubular liquid chromatography are two examples of an emerging area of analytical instrumentation known as microcolumn separations. The high resolution and small sample requirements of these methods make them suitable for the quantitative, multicomponent chemical analysis of single cells. Appropriate instrumentation for the analysis of nanoliter and subnanoliter samples is discussed. Data from the analysis of individual neurons are presented, including amino acid and neurotransmitter content.Keywords
This publication has 51 references indexed in Scilit:
- Optimization of a coaxial continuous flow fast atom bombardment interface between capillary liquid chromatography and magnetic sector mass spectrometry for the analysis of biomoleculesAnalytical Chemistry, 1989
- Capillary zone electrophoresis for the determination of electrophoretic mobilities and diffusion coefficients of proteinsJournal of Microcolumn Separations, 1989
- High sensitivity laser induced fluorescence detection in capillary zone electrophoresisJournal of High Resolution Chromatography, 1988
- Pneumatic microsyringe for use as an injector in open tubular liquid chromatography and as a dispenser in microanalysisAnalytical Chemistry, 1988
- New method for the preparation of small diameter columns with polymeric stationary phases for open tubular liquid chromatographyJournal of High Resolution Chromatography, 1988
- Improvements in scanning voltammetric detection for open-tubular liquid chromatographyAnalytical Chemistry, 1986
- Comments on improvement of the limit of detection in chromatography by an integration methodAnalytical Chemistry, 1986
- Scanning on-column voltammetric detector for open-tubular liquid chromatographyAnalytical Chemistry, 1986
- Chromatographic and Histochemical Identification of Dopamine Within an Identified Neuron in the Leech Nervous SystemJournal of Neurochemistry, 1983
- Endogenous levels of acetylcholine and choline in individual neurons of AplysiaJournal of Neurochemistry, 1973