Nucleic Acids and Their Derivatives in Alcoholic Extracts of Rat Tumors and Normal Muscle.

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Abstract
Spectral absorption analyses on alcohol extracts of tumors revealed a higher specific density/mg of nucleic acid than did similar extracts of normal muscle or than pure DNA or RNA. This high specific density, as well as type absorption curve, suggested the presence of guanosine or deoxyguanosine. Paper electrophoresis experiments, followed by study of paper strips for the position of ultraviolet-absorbing components, and for presence of ribosides by an orcinol spray, or of deoxyribosides by cysteine: H2SO4 spray, led to the conclusion that tumor extracts contained chiefly deoxyribosides, while muscle extract contained ribosides. This supposition was supported by chemical analyses and by highly specific bioassay methods. These methods indicated that the relative amounts of purine deoxyribosides and pyrimidine deoxyribosides in tumor extracts differed from those in normal muscle extracts.