Effect of Graded Levels of Insulin on Lactation in the Rat.

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Abstract
Summary The daily subcutaneous injection of 3 units of protamine zinc insulin into Sprague-Dawley-Rolfsmeyer lactating rats from day 7 to 19 stimulated highly significant increases in milk yield in comparison with the control group. On day 14 milk yield was increased 38%, on day 16, 25%, on day 18, 26%, and on day 20, 79%. The increase in mean litter weight in comparison with the controls was not significant. The mammary glands as measured by DNA were not significantly increased on day 20 by insulin.