Glycated Haemoglobin and Glycated Albumin: Evaluation of Different Methods in Diabetic Control

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Abstract
The levels of glycated Hb and albumin were determined in 56 diabetic patients aged between 5 and 19 yr. The glycohemoglobin levels were measured by 2 different methods, cation-exchange chromtogrpahy and a colorimetric chemical method. The glycated albumin levels were measured by a chemical method in plasma albumin purified with Affi-gel Blue. The results were compared with other indicators such as blood glucose and urinary glucose. The postprandial blood glucose levels were well correlated with the levels of glycoproteins: glycohemoglobin by the column method (r = 0.886), glycohemoglobin by the chemical method (r = 0.72) and glycoalbumin (r = 0.662). The urinary levels of glucose showed a weak correlation with blood glucose levels (r = 0.214) and with glycoalbumin (r = 0.298), but no correlation with glycohemoglobin. Significant correlations between the changes of glycated albumin levels and glycohemoglobin were found (r = 0.78). The result of the comparison of the chemical method with the column method for glycohemoglobin determination showed a high correlation between the values (r = 0.95). There were also significant differences in glycated protein levels between normal and diabetics. There were also significant differences in glycohemoglobin and glycoalbumin values between fairly managed and poorly managed groups of diabetics. Of the 3 parameters, glycoalbumin, glycohemoglobin and plasma glucose, the greatest absolute changes were found in the levels of glycoalbumin.