Intracellular cAMP regulates the response to NMDA in hippocampal neurons
- 1 November 1991
- journal article
- research article
- Published by Wolters Kluwer Health in NeuroReport
- Vol. 2 (11) , 673-676
- https://doi.org/10.1097/00001756-199111000-00010
Abstract
WE have studied the effects of intracellular cyclic AMP (cAMP) on the response to N-methyl-D-aspartate (NMDA) in hippocampal cultured neurons by loading them with 2'-o-dibutyryladenosine 3', 5'-cyclic monophosphate (dcAMP) and have obtained evidence for regulation of Ca2+ release from intracellular stores by cAMP. Extracellular Ringer's solution was either Ca2+-containing or Ca2+-free. A brief initial stimulation with NMDA(10–100 μM, 12 s) was required before the neurons were loaded with dcAMP to potentiate the changes in intracellular Ca2+ concentration induced by the second stimulus of NMDA. Forskolin (10 μM) mimicked dcAMP, but to a lesser extent. A phorbol ester 12-o-tetra-decanoylphorbol-13-acetate (100 ±M) inhibited the effect of dcAMP.Keywords
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