Complement-fixing immunoglobulin M antibody response in patients with infantile gastroenteritis

Publisher Website
Google Scholar
Abstract
Complement[C]-fixing immunoglobulin M antibody [Ab] to infantile gastroenteritis virus (a rotavirus) was detected with highest sensitivity when the Ab-antigen-C mixture was incubated at 37.degree. C for 1 h prior to the addition of sensitized sheep erythrocytes. Sucrose gradient centrifugation of sera collected sequentially from 4 patients after infection detected 19S C-fixing Ab up to 5 wk, with highest titers at 1 wk after the onset of illness. Treatment of the whole sera with 2-mercaptoethanol decreased C-fixing titers up to 2 wk after onset of illness.