Identification of a cDNA clone for mouse apoprotein A-1 (apo A-1) and its use in characterization of apo A-1 mRNA expression in liver and small intestine.

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Abstract
A c[complementary]DNA clone for mouse apoprotein A-1 (apo A-1), the major apoprotein of plasma high density lipoproteins, was identified. In addition to structural and physiological evidence, a genetic polymorphism for mouse plasma apo A-1 was used to confirm that this DNA sequence corresponds to the apo A-I gene. Use of this clone in molecular hybridization studies showed that the concentration of apo A-1 mRNA is similar in liver and small intestine and is constant along the entire length of the small intestine. The same apo A-I gene is expressed in both liver and small intestine. Apo A-1 mRNA is also present in the stomach and esophagus at 10-15% the concentration found in small intestine but is undetectable in other tissues (such as large intestine, pancreas, heart, kidney, spleen and brain). There is a differential effect of a diet high in saturated fat and cholesterol on apo A-1 mRNA levels in liver and small intestine.