SYNTHESIS OF ACYL PHOSPHATIDYLGLYCEROL FROM PHOSPHATIDYLGLYCEROL IN ESCHERICHIA-COLI K-12 - EVIDENCE FOR PARTICIPATION OF DETERGENT-RESISTANT PHOSPHOLIPASE-A AND HEAT-LABILE MEMBRANE-BOUND FACTOR(S)

  • 1 January 1978
    • journal article
    • research article
    • Vol. 528  (1) , 107-118
Abstract
The conversion of phosphatidylglycerol to acyl phosphatidylglycerol by extracts of E. coli K-12 strains was examined under various conditions. The maximum rate of conversion was observed at pH 7.2 in the presence of 50% (vol/vol) diethyl ether and 10 mM CaCl2. This conversion involved 2 sequential reactions: the formation of 2-acyl glycerophosphoglycerol and 2-acyl glycerophosphoethanolamine from phosphatidylglycerol and phosphatidylethanolamine, respectively, by detergent-resistant phospholipase A in the presence of Ca2+ and transfer of the acyl group of 2-acyl lysophospholipid to phosphatidylglycerol by a heat-labil factor(s) in the presence of diethyl ether. Neither fatty acid, acyl-CoA nor 1-acyl lysophospholipid could act as an acyl donor for phosphatidylglycerol. The heat-labile factor(s) was found in the inner membrane and supernatant fractions.

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