Modified Automated Determination of 2,3-Diphosphoglycerate in Whole Blood
Open Access
- 1 September 1972
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 18 (9) , 1001-1004
- https://doi.org/10.1093/clinchem/18.9.1001
Abstract
A modification is described of the automated determination of 2,3-diphosphoglycerate (DPG) in blood [Grisolia, S., et al., Anal. Biochem. 31, 235 (1969)]. Modifications in the manifold result in a sensitive, noise-free, rapid system and the modifications in the preparations of the reagents ensure stability of the diluted standards and blood samples for at least three weeks. Samples are run at the rate of 60/h and sample size can be as small as 5 µl of whole blood. The coefficient of variation of the overall determination of automated DPG and manual hemoglobin is 3.6% and the SD is ±0.77 µmol/g Hb. The normal range is 14.6 ± 2.2 (SD) µmol/g hemoglobin.Keywords
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