Enhancement of Resistance of Mice to Tuberculosis by Purified Components of Mycobacterial Lipid Fractions

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Abstract
Chemical heterogeneity and immunogenicity of lipid fractions of Mycobacterium tuberculosis and Mycobacterium bovis were examined by centrifugal chromatography through microparticulate silica gel and by tests of protection in mice challenged by the airborne route with M. tuberculosis H37Rv. Chromatography with solvents of low polarity revealed extreme heterogeneity of chloroform extracts, waxes B, C, and D, and cord factor prepared by standard procedures and of methanol and chloroform-methanol extracts. However, all preparations that had protective activity when combined with inactivated cell walls contained material that was immobile on the silica gel in weakly polar solvents. This immobile material yielded four purified components after preparative silica-gel chromatography in a solvent of higher polarity. Two of these components stimulated in mice a granulomatous response and high levels of resistance to airborne infection with tubercle bacilli.

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