ENZYMIC METHOD FOR SPECTROPHOTOMETRIC DETERMINATION OF OXALIC-ACID
- 1 January 1976
- journal article
- research article
- Vol. 87 (5) , 903-908
Abstract
A simple method is described for the measurement of [human] urinary oxalate. Oxalate decarboxylase [from Collybia velutipes] is coupled with NAD+ requiring formate dehydrogenase [from Pseudomonas oxalaticus] and the result recorded spectrophotometrically. Accurate determination can be carried out either on urine or a citrate extract of urine. Using the citrate extract procedure, the urinary samples can be stored for at least 3 mo. without any effect on the oxalate content.This publication has 3 references indexed in Scilit:
- Triton X-100 scintillant for carbon-14 labelled materialsThe International Journal of Applied Radiation and Isotopes, 1968
- Enzymatic Oxalate Determination in UrineClinical Chemistry, 1963
- ENZYMATIC DECARBOXYLATION OF OXALIC ACIDJournal of Biological Chemistry, 1957