Enzymatic Hydrolysis of Allergens and Assessment of the Remaining Allergenic Activities by the Paper Disc Radioallergosorbent Method
- 1 January 1973
- journal article
- Published by S. Karger AG in International Archives of Allergy and Immunology
- Vol. 45 (3) , 417-428
- https://doi.org/10.1159/000231059
Abstract
Birch-pollen and dog-epithelium allergenic extracts were hydrolyzed by the proteolytic enzyme pronase. The remaining allergenic activity in the hydrolyzed extract was then tested with various sera from allergen-sensitive patients, using the paper disc radioallergosorbent test method. The effect of enzymatic hydrolysis on dose-response curves was established. The isoelectrofocusing patterns of hydrolyzed and non-hydrolyzed allergen extracts were also compared.Keywords
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