An improved separation of aflatoxins

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Abstract
Crude aflatoxin from a chloroform extract ofAspergillus flavus cultures on rice was precipitated with Skelly Solve B and chromatographed on 100–200 mesh silica gel columns, using ethyl acetate as eluant. On this column there was no separation of aflatoxins from each other, but most of the brown, oily material was removed. The next step in the purification was chromatography on 100–200 mesh silica gel columns with chloroform and 5% methanol/chloroform as eluants. A large part of the B1 was purified, but B2, G1 and G2 did not separate, and M1 had a brown oil that prevented crystallization. The M1 was purified by chromatography on Sephadex LH‐20 with chloroform; the brown material was retained while the M1 passed through. The separation of aflatoxin B2, G1, and G2 was achieved by column chromatography on Silica Gel H for TLC. In addition, aspertoxin was separated and identified. The purity and identity of the compounds were established by 100 MHz NMR.