Biochemical Characterization of TEM-92 Extended-Spectrum β-Lactamase, a Protein Differing from TEM-52 in the Signal Peptide
Open Access
- 1 December 2002
- journal article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 46 (12) , 3981-3983
- https://doi.org/10.1128/aac.46.12.3981-3983.2002
Abstract
A bla TEM-92 gene was cloned from a Proteus mirabilis isolate and expressed in Escherichia coli . Production of the enzyme caused reduction of susceptibility to penicillins and narrow- to expanded-spectrum cephalosporins but not to moxalactam and cephamycins. Determination of kinetic parameters with the purified enzyme revealed hydrolysis of expanded-spectrum cephalosporins, while cephamycins, moxalactam, and aztreonam were very poorly or not hydrolyzed. Clavulanate and penicillanic acid sulfones acylated TEM-92 slowly, and deacylation occurred at measurable rates.Keywords
This publication has 17 references indexed in Scilit:
- Characterization of a New Extended-Spectrum β-Lactamase (TEM-87) Isolated in Proteus mirabilis during an Italian SurveyAntimicrobial Agents and Chemotherapy, 2002
- Extended-Spectrum β-Lactamases in the 21st Century: Characterization, Epidemiology, and Detection of This Important Resistance ThreatClinical Microbiology Reviews, 2001
- New TEM Variant (TEM-92) Produced by Proteus mirabilis and Providencia stuartii IsolatesAntimicrobial Agents and Chemotherapy, 2001
- A secondary drug resistance mutation of TEM-1 β-lactamase that suppresses misfolding and aggregationProceedings of the National Academy of Sciences, 2000
- A secondary drug resistance mutation of TEM-1 beta -lactamase that suppresses misfolding and aggregationProceedings of the National Academy of Sciences, 2000
- Extended-Spectrum TEM- and SHV-Type β-Lactamase-Producing Klebsiella pneumoniae Strains Causing Outbreaks in Intensive Care Units in ItalyEuropean Journal of Clinical Microbiology & Infectious Diseases, 2000
- EXTENDED-SPECTRUM β-LACTAMASES AND OTHER ENZYMES PROVIDING RESISTANCE TO OXYIMINO-β-LACTAMSInfectious Disease Clinics of North America, 1997
- Survey of prevalence of extended spectrum β-lactamases among EnterobacteriaceaeJournal of Antimicrobial Chemotherapy, 1996
- TEM β-Lactamase Mutants Hydrolysing Third-generation Cephalosporins: A Kinetic and Molecular Modelling AnalysisJournal of Molecular Biology, 1994
- Automated analysis of enzyme inactivation phenomenaBiochemical Pharmacology, 1987