SUPPRESSION OF TRANSFERRED ALLOGENEIC PLAQUE-FORMING CELLS IN MICE

Publisher Website
Google Scholar
Abstract
Spleens of mice which were immunized with sheep or horse red blood cells contained a population of hemolysin-producing cells that could be detected with the Jerne plaque assay. Plaque-forming cells (PFCs) were detected up to 3 days after adoptive transfer into the peritoneal cavities of recipient histoincompatible mice. When the recipient mice were sensitized by i.p. injection of cells syngeneic with the donor of the transferred cells at least 72 hr prior to transfer, the hemolytic plaque response of the transferred cells was largely suppressed. Early suppression was limited to the peritoneal cavity; allografted spleen cells were consistently detected in the spleens of recipient mice up to 3 days after transfer whether sensitized or not. The suppression response was specific for the allospecificities against which recipient mice were sensitized. The response was also proportional to the dose of sensitizing cells between 0.1 and 5 X 106 cells and was linear with the log10 of the cell number used for sensitization plotted against log10 number of plaques/mouse. When two populations of spleen cells, labeled by immunization to horse or sheep red blood cells, respectively, from non-cross reacting target allotypes were individually transferred to unsensitized recipients, each population was detected by its independent label. If recipient mice were sensitized to either allotype, the cells with the non-cross reacting alloantigen (the innocent bystanders) were also suppressed when mixed with the specific target cells.

This publication has 0 references indexed in Scilit: