Labeled Iron Stores in Study of Iron Liberated During Red Cell Destruction by Acetylphenylhydrazine

Publisher Website
Google Scholar
Abstract
A study of the fate of Fe liberated during red cell destruction was made using dogs whose Fe stores were labeled with Fe59 by the intraven. injns. into dogs whose hematological picture indicated adequate Fe in the various Fe compartments. At the completion of the injns. of Fe59, it was calculated that there should be 285 mg. of Fe in storage of which 50% was Fe59. The dogs were then given 400 mg. of acetylphenylhydrazine intraven. which resulted in total destruction of the original red cells within 10 days. If the Fe liberated from the red cells went to the storage sites and mixed with the storage Fe, there would then be 825 mg. of Fe present in storage and 14% of this would be Fe59. If the storage Fe was used indiscriminately for the synthesis of new cells, the activity of the red cells when their volume reached normal values would have indicated an uptake of about 50% of the Fe59. as the max. uptake observed was only 4.2% it was concluded that the red cell Fe was preferentially used for the synthesis of new cells. This same phenomenon was observed in an animal which had not been subjected to acetylphenylhydrazine.