A Convenient Radiometric Assay for Hyaluronan

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Abstract
Hyaluronan-binding proteins (HABP) isolated from bovine cartilage have been utilized in a radiometric assay for determination of hyaluronan (hyaluronic acid; HA) in serum. The samples to be analysed are preincubated with iodinated HABP. After 60 minutes the protein that is not bound to hyaluronan, in the solution, is recovered on HA-Sepharose in a 45-min incubation. Hyaluronan can be determined in the range from 10 to 1000 μg/1. The sample size is 100 μ1 and the intra-assay and inter-assay coefficients of variations are < 10% and < 10%, respectively for samples containing hyaluronan concentrations in the range 10–200 μg/1. Detection limit is 4 μg/1.