Enzymic comparisons of the inorganic sulfur metabolism in autotrophic and heterotrophic Thiobacillus ferrooxidans

Abstract
Activities of enzymes which mediate the oxidation of thiosulfate to sulfate and the assimilation of sulfate to sulfide were assayed in various cell-free fractions of T. ferrooxidans grown autotrophically on either Fe2+ or S2O32- or heterotrophically on glucose. There was no activity of the thiosulfate-oxidizing enzyme in extracts of bacteria grown with Fe2+. Comparable activities for ATP-sulfurylase (EC 2.7.7.4), ADP-sufrylase (EC 2.7.7.5) and adenylate kinase (EC 2.7.4.3) were found in the bacteria grown autotrophically with either Fe2+ or S2O32- or heterotrophically with glucose.

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