Efficiency of isolation of revertible 6-thioguanine resistant clones is dependant on the concentration of thioguanine used for selection

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Abstract
The availability of a number of well characterised mammalian cell mutants which revert at measurable frequencies when exposed to specific chemical mutagens would considerably aid analysis of mechanisms of mutagenesis in mammalian cells, might ultimately provide the mammalian cell equivalent of the ‘Ames’ tester strains and could aid the understanding of the spectrum of mutations produced by different carcinogens. As an approach to this goal we have analysed the phenotypes and revertibility of a considerable number of 6-thioguanine resistant (6TGR mutants isolated from V79 cells and have determined the 6TG concentration which yields the optimum number of revertible cell lines.